We found that enhancers DlxI12b, 692, and 1538 are active in Lh圆-GFP + cells, while enhancer 1056 is active in Olig2 + cells.
Here we demonstrate the utility of enhancer elements as tools to mark MGE-like cells in ES cell differentiation experiments. We hypothesized that enhancers that are active in the mouse MGE would be useful tools in detecting when ES cells differentiate into MGE cells. Using a modified embryoid body method, we provided gene expression evidence that mouse ES-derived Lh圆 + cells closely resemble immature interneurons generated from authentic MGE-derived Lh圆 + cells. Herein, we describe approaches to generate MGE-like progenitor cells from mouse embryonic stem (ES) cells. MGE transplantation into specific regions of the postnatal central nervous system modifies circuit function and improves deficits in mouse models of epilepsy, Parkinson's disease, pain, and phencyclidine-induced cognitive deficits.
The medial ganglionic eminence (MGE) is an embryonic forebrain structure that generates the majority of cortical interneurons.
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